W.T. Evert Ting, Ph.D.

W. Evert Ting

Office: Gyte 295
Telephone: (219) 989-2490
Email: ting@purduecal.edu

Education

Ph.D. Microbiology, The Ohio State University, Columbus, Ohio,1986
M.S. Microbiology, The Ohio State University, Columbus, Ohio, 1983
B.S.A. Plant Pathology, National Taiwan University, Taipei, Taiwan, 1978.

Research Interests

Bacterial phages have been found useful to treat infectious diseases. Recent studies indicated that phages could be used as bio-preservatives to control foodborne pathogens. We have isolated several phages from raw sewage samples that can cause lyses of E. coli O157:H7 and Salmonella. We continuously isolate and select such phages and test various combinations of phages to control the target bacteria in spiked food samples.

Polycyclic aromatic hydrocarbons (PAHs) are a group of organic lipophilic pollutants generated during incomplete combustion of organic carbons such as burning fossil fuels, wood, and municipal solid waste. They are of great environmental concern because of their ubiquitous presence and persistence in air, water, and soil, as well as their toxicity to humans and other living organisms. We are studying the degradation of PAHs using an edible white rot fungus, Ganoderm lucidum.

Escherichia coli is a common fecal indicator used to monitor food and water quality. High E. coli counts in water signifying fecal contamination often leads to beach closure. We have used DNA fingerprinting techniques such as randomly amplified polymorpohic DNA (RAPD) and automated ribotyping techniques for tracing sources of E. coli in recreational waters and studying the diversity of E. coli persisted in beach sand. We have built a database consists of hundreds of RAPD fingerprints and Riboprints of E. coli from both human and nonhuman sources for E. coli source tracking.

Hydrogen is considered as a clean energy source, which can be used for generating electricity without causing air pollution. There are increased interests of using microbes to convert organic wastes to hydrogen. We isolated and selected microbes from environment samples that can produce hydrogen from organic wastes.  We studied the optimal conditions for hydrogen production.

Professional Society Memberships

American Society for Microbiology (ASM). 1979 – Present
Institute of Food Technologist (IFT). 1989 – Present
Chinese American Society for Microbiology(CASM) 2000 – Present

Publications:

W.T. E. Ting a, S.Y. Yuan b, S.D. Wu c, B.V. Chang. 2011. Biodegradation of phenanthrene and pyrene by Ganoderma luciden. International Biodeterioration & Biodegradation 65:238-242.

Kramer, R., Pelter, L., Patterson, J., Kmiotek, K., Ting, E. and J. Patterson. 2011. Modular waste/ renewable energy system for production of electricity, heat, and potable water in remote locations. Proceedings of 2011 IEEE Global Humanitarian Technology Conference, p.30-35

W.-T. E. Ting, S. Susoreny, L. S. Pelter, and R. Kramer, 2008. “Production of Hydrogen by Dark Fermentation of Food Waste” Abst. O-012. In Abstracts of the 105th General Meeting of the American Society for Microbiology, American Society for Microbiology, Boston, MA.

M. N. Byappanahalli, R. L. Whitman, D. A. Shively, W. T. Evert Ting, C. C. Tseng, and M. B. Nevers. 2006. Seasonal persistence and population characteristics of Escherichia coli and enterococci in deep backshore sand of two freshwater beaches. Journal of Water and Health 04:313-320.

Ting, W.T.E., D. S. Johnson, M. N. Byappanahalli, R. L. Whitman, C. C. Tseng. 2005. Genetic Diversity of Escherichia coli isolated from backshore sand of two freshwater beaches of southern Lake Michigan. Abst. Q326. In Abstracts of the 105th General Meeting of the American Society for Microbiology, American Society for Microbiology, Washington D.C.

Ting, W.T.E., D.S. Johnson, and C. C. Tseng. 2004. A comparison of riboprint patterns of Escherichia coli isolated from cows, deer, horses, and sewage in two different geographic areas. Abst. Q247.  In Abstracts of the 104th General Meeting of the American Society for Microbiology, American Society for Microbiology, Washington D.C.

Ting, W.T.E., D.S. Johnson, A.M. Holler, K.T. Tran, and C.C. Tseng. 2003. A Study of the sources of E. coli contamination at Marquette Park Beach by random amplified polymorphic DNA typing. Abstr. Q414, p.589  In Abstracts of the 103th General Meeting of the American Society for Microbiology, American Society for Microbiology, Washington D.C.

Ting, W.T.E., D.S. Johnson, G.A. Thomas, A.M. Holler, and C.C. Tseng. 2002. Discriminant analysis of random amplified polymorphic DNA (RAPD) patterns of Escherichia coli isolated from different human and nonhumean sources. Abstr. Q108 , p.396 In Abstracts of the 102th General Meeting of the American Society for Microbiology, American Society for Microbiology, Washington D.C.

Tseng, C.C., D. Johnson, and W.T. E. Ting. 2002. Comparison of ribotypes of Escherichia coli from sewage, humans, and animals. abstr.Q-59, p.388. In Abstracts of the 102th General Meeting of the American Society for Microbiology, American Society for Microbiology, Washington D.C.

Tseng, C.C, D.J. Johnson, and W.T.E. Ting. 2002. Differentiation of E coli isolated from human and nonhuman sources using automated ribotyping method. In Proceeding of 2002 Great Lakes Beach Conference, Chicago.

Ting, W.T.E., J. Vander Hoogt and R. Whitman. 2001. Growth and survival of Escherichia coli O157:H7 and a lake water Escherichia coli isolate in wet beach sand and water from Lake Michigan. Abstr. Q364, p.658. In Abstracts of the 101th General Meeting of the American Society for Microbiology 2001, American Society for Microbiology, Washington D.C.

Tseng, C, E. Ting, D. Johnson, M. Saluta, and R. Dunst. 2001. RAPD fingerprinting as a potential means for differentiating human and animal E. coli. Life Sci. News 7:10-11.

Tseng, C. C. D. Johnson, and W. T. E. Ting. 2001. Automated ribotyping of Escherichia coli isolates from human and animals. Abstr. Q-330, p.650. In Abstracts of the 101th General Meeting of the American Society for Microbiology 2001, American Society for Microbiology, Washington D.C.

Ting, W.T.E., C. C. Tseng, D. S. Johnson, L. Dominguez, J. Vander Hoogt, M. Saluta, R. L. Whitman. 2000. Genetic Diversity of Escherichia coli Isolated from Lake Michigan Water, Beach Sand, and Seagull Droppings as Revealed by Random Amplified Polymorphic DNA Fingerprints. abstr. Q-305, p.610-611. In Abstracts of the 100th General Meeting of the American Society for Microbiology 2000, American Society for Microbiology, Washington D.C.

Tseng, C. C., W. T. E. Ting, D. Johnson, L. D.Dominguez, J. Vandor Hoodgt, K. Davis-Fleisher, and M. Saluta. 2000. Efficacy of RAPD analysis for differentiating human from animal E. coli isolates. abstr. Q-304, p.610. In Abstracts of the 100th General Meeting of the American Society for Microbiology 2000, American Society for Microbiology, Washington D.C.

Tseng, C. C., W. T. E. Ting, Y. Cheng, D. Johnson, and M. Saluta. 1999.  A comparative study of RAPD fingerprints of Escherichia coli isolates from humans and animals. . abstr. Q-69, p.546. In Abstracts of the 99th General Meeting of the American Society for Microbiology 1999, American Society for Microbiology, Washington D.C.

Koonce, M. and W. T. E. Ting. 1998.  Effect of Oxyraseâ on the recovery of bifidobacteria in untreated waste water.  abstr. Q-132, p.443. In Abstracts of the 98th General Meeting of the American Society for Microbiology 1998, American Society for Microbiology, Washington D.C.

Saluta, M, W. T. E. Ting, M. Koonce and C. C. Tseng. 1998. Cluster analysis of RAPD fingerprints of Escherichia coli isolates from human stool, urine, and blood.  abstr. H-124, p.297. In Abstracts of the 98th General Meeting of the American Society for Microbiology 1998, American Society for Microbiology, Washington D.C.

Tseng, C. C., M. Koonce, W. T. E. Ting, and M. Saluta. 1998.  Comparison of RAPD fingerprints of Escherichia coli isolates from human and Canadian geese.  abstr. R-2, p.480. In Abstracts of the 98th General Meeting of the American Society for Microbiology 1998, American Society for Microbiology, Washington D.C.

Ting, W.T. E., E. M. Nielson, and C.C. Tseng. 1997. The use of Viraffinity matrix to concentrate waterborne polioviruses for RT-PCR detection. abstr. Q-169. p.484. In Abstracts of the 97th General Meeting of the American Society for Microbiology 1997, American Society for Microbiology, Washington D.C.

Tseng, C. C., W. T. E. Ting and M. Sunata. 1997.  Random amplified polymorphic DNA (RAPD) analysis of dynamic human Escherichia coli genome. abstr. H-220. p.321. In Abstracts of the 97th General Meeting of the American Society for Microbiology 1997, American Society for Microbiology, Washington D.C.

Ting, W. T. E., M. Saluta, and C. C. Tseng. 1997. Tracking sources of Escherichia coli in water. p.66-73. In Proceedings of A National Healthy Beaches Symposium, Research, Monitoring and Cooperative Efforts.  Chesterton, IN. Illinois-Indiana Seagrant Collage Program.

Ting, W. T. E., D. Johnson, D. George, and D. Warner. 1996.  Comparing three selective media for enumeration of bifidobacteria in untreated waste water. abstr., Q-451 p.464. In Abstracts of the 96th General Meeting of the American Society for Microbiology 1996, American Society for Microbiology, Washington D.C.

Ting, W. T. E., E. Nielson, K. M. Zmuda, K. Hedges, and C. C. Tseng. 1996.  RT-PCR detection of poliovirus from water.  The 112th Annual Meeting of Indiana Academy of Science. Programs and Abstracts, p.74-75.

Ting, W. T. E. 1993.  Effect of food grade phosphate on the growth of Listeria monocytogenes. 1993 IFT Annual Meeting Technical Program: Book of Abstracts, Paper No. 656, p.166.

Ting*, W. T. E. and K. E. Deibel.  1992. Sensitivity of Listeria monocytogenes to spices at two temperatures.  Journal of Food Safety 12:129-137.

Ting, W. T. E. and J. G. Alb, Jr.  1991. Combined effect of spices and lactic acid on the growth and survival of Listeria monocytogenes1991 IFT Annual Meeting Program and Food Expo Exhibit Directory, Paper No. 523, p. 216.

Alb, J. G. Jr., D. L. Cansler, and W. T. E. Ting. 1990.  Effect of sage on the growth and survival of Listeria monocytogenesAbstracts of the Annual Meeting of the Indiana Branch of American Society for Microbiology.  p. 16.

Alb*, J. G. Jr. and W. T. E. Ting. 1990.  Inhibition of Listeria monocytogenes by food grade phosphates. Abstracts of the Annual Meeting of the Indiana Branch of American Society for Microbiology. p. 16.

Tseng, C, E. Ting, D. Johnson, M. Saluta, and R. Dunst.  2001. RAPD fingerprinting as a potential means for differentiating human and animal E. coli.  Life Sci. News 7:10-11.

Ting*, W. T. and G. J. Banwart.  1985. Detection of Bacillus cereus diarrheagenic toxin using a rat intestinal loop assay.  Journal of Food Safety 7:57-63.

Ting*, W. T. and G. J. Banwart.  1985. Enumeration of enterococci and mesophilic aerobic plate count in dried soup using three reconstitution methods.  Journal of Food Protection 48:770-771.

Courses

  • BIOL 10700 —Freshman Experience in Biological Sciences
  • BIOL 22100 — Introductory Microbiology 
  • BIOL 31600 — Basic Microbiology 
  • BIOL 53300 — Medical Microbiology 
  • BIOL 53400 — Medical Microbiology Lab 
  • BIOL 59500 — Food Microbiology 
  • BIOL 48900, 49500 and 59500 — Microbiology Research 
  • BIOL 59500 — Microbiology Readings
  • BIOL 59500 — Environmental Microbiology
  • BIOL 69800 — Research MS Thesis