Subject: BIOL 50800 Recombinant DNA Techniques
Credits: 3 (Class 1, Lab 5)
Offered: Every 2 years
Description: Basic principles of genetic engineering and gene cloning with various vectors. Techniques include isolation of DNA, use of restriction endonucleases, separation of DNA fragments, transformation of E. coli with recombinant DNA, detection of DNA sequences in Southern blot hybridization, mRNA isolation, cDNA library construction, DNA sequencing, and PCR technology.